SMAD9 antibody (Internal Region)

Catalog No. ABIN499162

Product Details anti-SMAD9 Antibody

Target: SMAD9 (SMAD Family Member 9 (SMAD9))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This SMAD9 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-HSEPLMPHNA TYPD

Specificity: This antibody recognizes SMAD9 and is not expected to cross-react with SMAD1 and SMAD5.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog).
Species reactivity (tested):Human

Purification: Ammonium Sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_005896.1

Application Details

Application Notes: Peptide ELISA: limit dilution 1: 64000. Western blot: 0.3-1.0 μg/mL, approx 50 kDa band observed in Human Skin lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SMAD9

Target: SMAD9 (SMAD Family Member 9 (SMAD9))

Alternative Name: SMAD9 (SMAD9 Products)

Synonyms: SMAD8 antibody, MADH6 antibody, MADH9 antibody, PPH2 antibody, SMAD8A antibody, SMAD8B antibody, Madh8 antibody, Madh9 antibody, Smad8 antibody, SMAD family member 9 antibody, SMAD9 antibody, smad9 antibody, Smad9 antibody

Background: SMAD9 is a member of the MAD-related family of molecules. MAD-related proteins are a recently identified family of intracellular proteins that are thought to be essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily.Synonyms: MAD homolog 9, MADH9, Mothers against DPP homolog 9, Mothers against decapentaplegic homolog 9, SMAD 9, SMAD family member 9, SMAD-9

Molecular Weight: 48.6 kDa (NP_005896.1)

Gene ID: 4093

NCBI Accession: NP_001120689

UniProt: O15198