XPNPEP1 antibody (C-Term)

Catalog No. ABIN499169

The Goat Polyclonal anti-XPNPEP1 antibody is suitable to detect XPNPEP1 in samples from Human, Mouse, Rat, Pig, Dog and Cow. It has been validated for WB.

Quick Overview for XPNPEP1 antibody (C-Term) (ABIN499169)

Target: XPNPEP1 (X-Prolyl Aminopeptidase (Aminopeptidase P) 1, Soluble (XPNPEP1))

Reactivity: Human, Mouse, Rat, Pig, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This XPNPEP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-XPNPEP1 Antibody

Binding Specificity: C-Term

Sequence: C-LIRETQPISK QH

Specificity: This antibody detects XPNPEP1 at C-term. It is expected to recognize isoform 1 and 2 (NP_065116.2, NP_001161076.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, rat, cow, pigSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_065116.2, NP_001161076.1

Application Details

Application Notes: Peptide ELISA: limit dilution 1: 32000. Western blot: 0.3-1 μg/mL. Approx 80 kDa band observed in Human Heart, Skeletal Muscleand Pancreas lysates (calculated MW of 69.0 kDa according to NP_065116.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer.

Target Details for XPNPEP1

Target: XPNPEP1 (X-Prolyl Aminopeptidase (Aminopeptidase P) 1, Soluble (XPNPEP1))

Alternative Name: XPNPEP1

Background: XPNPEP1 is a proline-specific metalloaminopeptidase that specifically catalyzes the removal of any unsubstituted N-terminal amino acid that is adjacent to a penultimate proline residue. As a result of its specificity toward proline, it has been suggested that XPNPEP1 is important in the maturation and degradation of peptide hormones, neuropeptides, and tachykinins, as well as in the digestion of otherwise resistant dietary protein fragments, thereby complementing the pancreatic peptidases. Deficiency of XPNPEP1 results in excretion of large amounts of imino-oligopeptides in urine.Synonyms: Aminoacylproline aminopeptidase, Cytosolic aminopeptidase P, Soluble aminopeptidase P, X-Pro aminopeptidase 1, X-prolyl aminopeptidase 1 soluble, XPNPEPL, XPNPEPL1, Xaa-Pro aminopeptidase 1

Gene ID: 7511

NCBI Accession: NP_065116

UniProt: Q9NQW7