AES antibody (C-Term, N-Term)

Catalog No. ABIN499225

This Rabbit Polyclonal antibody specifically detects AES in WB, IF and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for AES antibody (C-Term, N-Term) (ABIN499225)

Target: AES (Amino-terminal Enhancer of Split (AES))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AES antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-AES Antibody

Binding Specificity: C-Term, N-Term

Specificity: AES antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human AES.

Purification: Affinity chromatography purified via peptide column

Immunogen: Human AES / Amino-terminal enhancer of split / GRG / ESP1 / TLE5 (C-Terminus) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: AES antibody can be used for the detection of AES at 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for AES

Target: AES (Amino-terminal Enhancer of Split (AES))

Alternative Name: AES / ESP1

Background: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (2,3). AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis (4). It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. (4). Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (4).Synonyms: AES1, Amino-terminal enhancer of split, GRG protein, Gp130-associated protein GAM

Gene ID: 166

NCBI Accession: NP_001121

UniProt: Q08117

Pathways: WNT Signaling, SARS-CoV-2 Protein Interactome