APP antibody (N-Term)

Catalog No. ABIN499319

The Rabbit Polyclonal anti-APP antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect APP in samples from Human, Mouse and Rat. There is 1 publication available.

Quick Overview for APP antibody (N-Term) (ABIN499319)

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-APP Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts to APP.

Purification: Affinity chromatography purified via peptide column

Immunogen: APP antibody was raised against an 18 amino acid peptide near the amino terminus of human APP.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: APP antibody can be used for detection of APP at 1 - 2 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

References for anti-APP antibody (ABIN499319)

Nikolaev, McLaughlin, OLeary, Tessier-Lavigne: "APP binds DR6 to trigger axon pruning and neuron death via distinct caspases." in: Nature, Vol. 457, Issue 7232, pp. 981-9, (2009) (PubMed).

Target Details for APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Alternative Name: Amyloid beta A4 Protein / APP

Background: Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by one of two beta-secretases (BACE and BACE2), producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the Alzheimer's disease patients' brains. Recently, Death Receptor 6 (DR6) was found to interact with an amino-terminal fragment of the Beta-amyloid protein (N-APP) in neurons, activating a caspase 6-dependent apoptotic event leading to axonal degeneration and pruning during development, suggesting that these two proteins are involved in neural development and may possibly play a role in Alzheimer's disease.Synonyms: ABPP, APPI, Alzheimer disease amyloid protein, Amyloid Precursor Protein, CVAP, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II

Gene ID: 1238255

UniProt: P05067

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour