NOL3 antibody (N-Term)

Catalog No. ABIN499329

This anti-NOL3 antibody is a Rabbit Polyclonal antibody detecting NOL3 in WB and EIA. Suitable for Human, Mouse and Rat.

Quick Overview for NOL3 antibody (N-Term) (ABIN499329)

Target: NOL3 (Nucleolar Protein 3 (Apoptosis Repressor with CARD Domain) (NOL3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOL3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-NOL3 Antibody

Binding Specificity: N-Term

Specificity: ARC antibody was raised against a peptide corresponding to amino acids 2 to 18 of human origin. These sequences are identical to those of human nuclear protein Nop30 (2) and differ from those of the rat homolog of ARC by one amino acid (3).

Purification: Affinity chromatography purified via peptide column

Immunogen: Human ARC (N-Terminus) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: ARC antibody can be used for detection of ARC at 1/500 dilution. Whole celllysate from HeLa cells can be used as positive control and an approximately 25 kDa bandcan be detected.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for NOL3

Target: NOL3 (Nucleolar Protein 3 (Apoptosis Repressor with CARD Domain) (NOL3))

Alternative Name: NOL3 / ARC

Background: Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK BCL10, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD (1). ARC interacts with caspase-2 and -8 and inhibits enzymatic activity of caspase-8. ARC suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1, and DR3. The messenger RNA of ARC is primarily expressed in skeletal muscle and cardiac tissue (1).Synonyms: Apoptosis repressor with CARD, Muscle-enriched cytoplasmic protein, Myp, NOP, Nop30, Nucleolar protein 3, Nucleolar protein of 30 kDa

Gene ID: 8996

NCBI Accession: NP_003937

UniProt: O60936