HUWE1 antibody (C-Term)

Catalog No. ABIN499331

The Rabbit Polyclonal anti-HUWE1 antibody has been validated for WB, IF and EIA. It is suitable to detect HUWE1 in samples from Human, Mouse and Rat.

Quick Overview for HUWE1 antibody (C-Term) (ABIN499331)

Target: HUWE1 (HECT, UBA and WWE Domain Containing 1, E3 Ubiquitin Protein Ligase (HUWE1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HUWE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-HUWE1 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects ARF-BP1 at C-term.

Purification: Affinity chromatography purified via peptide column

Immunogen: A 19 amino acid peptide from near the carboxy terminus of Human ARF-BP1

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: ARF-BP1 antibody can be used for detection of ARF-BP1 at 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HUWE1

Target: HUWE1 (HECT, UBA and WWE Domain Containing 1, E3 Ubiquitin Protein Ligase (HUWE1))

Alternative Name: HUWE1 / UREB1

Background: The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53, this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.Synonyms: ARF-BP1, ARF-binding protein 1, E3 ubiquitin-protein ligase HUWE1, HECT, HSPC272, HectH9, Homologous to E6AP carboxyl terminus homologous protein 9, KIAA0312, KIAA1578, LASU1, Large structure of UREB1, Mcl-1 ubiquitin ligase E3, Mule, UBA and WWE domain-containing protein 1, URE-B1, URE-binding protein 1, Upstream regulatory element-binding protein 1

Gene ID: 10075

NCBI Accession: NP_113584

UniProt: Q7Z6Z7