ASAH2 antibody (C-Term)

Catalog No. ABIN499341

The Rabbit Polyclonal anti-ASAH2 antibody (ABIN499341) specifically detects ASAH2 in WB and EIA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ASAH2 antibody (C-Term) (ABIN499341)

Target: ASAH2 (N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASAH2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ASAH2 Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to ASAH2.

Purification: Affinity chromatography purified via peptide column

Immunogen: ASAH2 antibody was raised against a 17 amino acid peptide near the carboxy terminus of the human ASAH2.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: ASAH2 antibody can be used for detection of ASAH2 at 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for ASAH2

Target: ASAH2 (N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2))

Alternative Name: Neutral Ceramidase / ASAH2

Background: Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5-8.5. ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed primarily expressed with higher levels in the intestine, kidney, skeletal muscle and heart. Recent studies indicate that ASAH2 encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract.Synonyms: Acylsphingosine deacylase 2, BCDase, HNAC1, LCDase, N-acylsphingosine amidohydrolase 2, Non-lysosomal ceramidase, hCD

Gene ID: 56624

NCBI Accession: NP_063946

UniProt: Q9NR71