BIK antibody (N-Term)

Catalog No. ABIN499467

The Rabbit Polyclonal anti-BIK antibody (ABIN499467) specifically detects BIK in WB, IF and EIA. The antibody is reactive with Human and Mouse samples.

Quick Overview for BIK antibody (N-Term) (ABIN499467)

Target: BIK (BCL2-Interacting Killer (Apoptosis-Inducing) (BIK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BIK antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-BIK Antibody

Binding Specificity: N-Term

Specificity: This anitbody reacts to BIK.

Purification: Affinity chromatography purified via peptide column

Immunogen: BIK antibody was raised against a 15 amino acid peptide from near the amino terminus of human BIK.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: BIK antibody can be used for the detection of BIK ot at 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for BIK

Target: BIK (BCL2-Interacting Killer (Apoptosis-Inducing) (BIK))

Alternative Name: BIK

Background: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such protein BIK was recently identified as an endoplasmic reticulum (ER)-residing pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family that stimulates mitochondrial release of cytochrome c following p53 induction of apoptosis. A significant fraction of BIK is found as an ER transmembrane protein, with most of the protein facing the cytosol. Restricting BIK to the ER membrane by replacing the transmembrane region with that of the ER-selective membrane anchor of cytochrome b(5) resulted in a decreased cytochrome c release from mitochondria and a corresponding drop in cell death. Recent evidence suggests that BIK cooperates with NOXA, another BH3-only protein, to somehow enhance the activation of Bax to stimulate the rapid release of cytochrome c from mitochondria.Synonyms: Apoptosis inducer NBK, BIP1, BP4, Bcl-2-interacting killer, NBK

Gene ID: 638

UniProt: Q13323

Pathways: Positive Regulation of Endopeptidase Activity