BUB3 antibody (C-Term, N-Term)

Catalog No. ABIN499513

This Rabbit Polyclonal antibody specifically detects BUB3 in WB, IF and EIA. It exhibits reactivity toward Human and Mouse.

Quick Overview for BUB3 antibody (C-Term, N-Term) (ABIN499513)

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-BUB3 Antibody

Binding Specificity: C-Term, N-Term

Specificity: This antibody reacts to Bub3.

Purification: Affinity chromatography purified via peptide column

Immunogen: Bub3 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human bub3.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: Bub3 antibody can be used for detection of bub3 at 0.5 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for BUB3

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Alternative Name: BUB3

Background: The mitotic checkpoint protein Bub3 is involved with the essential spindle checkpoint pathway which operates during early embryogenesis. Bub3 is important during G2 and early mitosis stages, permitting entry into mitosis depending upon the assembly state of microtubules, thus preventing premature sister chromatid separation, mis-segregation and aneuploidy. Bub3 contains four WD repeat domains and is required for the kinetochore localization of Bub1, a related kinase that is necessary for spindle assembly checkpoint function. Bub1 is able to autophosphorylate and can catalyze the phosphorylation of Bub3. Both Bub1 and Bub3 are mutually dependent for function. Altered Bub expression levels may significantly impair mitotic checkpoint function and is associated with tumor cell proliferation.Synonyms: Mitotic checkpoint protein BUB3

Gene ID: 9184

UniProt: O43684

Pathways: Maintenance of Protein Location