PLEKHO1 antibody (C-Term)

Catalog No. ABIN499637

The Rabbit Polyclonal anti-PLEKHO1 antibody is suitable to detect PLEKHO1 in samples from Human, Mouse and Rat. It has been validated for WB and EIA.

Quick Overview for PLEKHO1 antibody (C-Term) (ABIN499637)

Target: PLEKHO1 (Pleckstrin Homology Domain Containing, Family O Member 1 (PLEKHO1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLEKHO1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PLEKHO1 Antibody

Binding Specificity: C-Term

Purification: Affinity chromatography purified via peptide column

Immunogen: CKIP1 antibody was raised against an 18 amino acid peptide from near the carboxy terminus of human CKIP1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: CKIP1 antibody can be used for detection of CKIP1 at 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for PLEKHO1

Target: PLEKHO1 (Pleckstrin Homology Domain Containing, Family O Member 1 (PLEKHO1))

Alternative Name: PLEKHO1 / CKIP1

Background: CKIP1 was identified through a yeast-two hybrid screening as a protein that would interact with only one of the two catalytic subunits of the casein kinase 2 complex. CKIP1 is a pleckstrin homology domain-containing protein localized within the nucleus and at the plasma membrane that interacts with CK2alpha but not CK2alpha', and is thought to play a role in targeting CK2alpha to a particular cellular location. CKIP1 has been implicated in muscle differentiation and the regulation of cell morphology and actin cytoskeleton. CKIP1 can also interact with other proteins such as ATM, an upstream kinase of p53, and recruit the nuclear ATM to the plasma membrane, suggesting CKIP1 may regulate ATM function through re-localizing ATM. CKIP1 can also form a complex with the kinase AKT, leading to a down-regulation of PI3K/AKT signaling and suppression of tumor growth in vivo.Synonyms: C-Jun-binding protein, CK2-interacting protein 1, CKIP-1, Casein kinase 2-interacting protein 1, HQ0024c, JBP, OC120, Osteoclast maturation-associated gene 120 protein, PH domain-containing family O member 1, Pleckstrin homology domain-containing family O member 1

Gene ID: 51177

NCBI Accession: NP_057358

UniProt: Q53GL0