CNOT4 antibody (N-Term)

Catalog No. ABIN499646

The Rabbit Polyclonal anti-CNOT4 antibody has been validated for WB and EIA. It is suitable to detect CNOT4 in samples from Human, Mouse and Rat.

Quick Overview for CNOT4 antibody (N-Term) (ABIN499646)

Target: CNOT4 (CCR4-NOT Transcription Complex Subunit 4 (CNOT4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNOT4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CNOT4 Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts to CNOT4.

Purification: Affinity chromatography purified via peptide column

Immunogen: CNOT4 antibody was raised against a 19 amino acid peptide near the amino terminus of the human CNOT4.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: CNOT4 antibody can be used for detection of CNOT4 at 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for CNOT4

Target: CNOT4 (CCR4-NOT Transcription Complex Subunit 4 (CNOT4))

Alternative Name: CNOT4

Background: CNOT4 is a component of the CCR4-NOT transcription complex, a complex that is implicated in the repression of RNA polymerase II transcription. In the CCR4-NOT complex, CNOT4 acts as an E3 ubiquitin-protein ligase and interacts with a subset of E2 ubiquitin-conjugating enzymes through a unique C4C4 RING domain. This E3 ligase activity was shown to be dependent on the selective and specific interaction with the ubiquitin conjugating enzyme UbcH5B. In yeast, mutations in CNOT4 that prevented its interaction with the UbcH5B homolog UBC4 caused increased sensitivity to hydroxyurea, heat shock, and hygromycin B, suggesting that CNOT4 and UbcH5B are involved in stress response in vivo. Multiple isoforms of CNOT4 are known to exist.Synonyms: CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4, E3 ubiquitin-protein ligase CNOT4, NOT4, NOT4Hp, Potential transcriptional repressor NOT4Hp

Gene ID: 4850

NCBI Accession: NP_037448

UniProt: O95628