CXCR4 antibody (Extracellular Loop)

Catalog No. ABIN499698

The Rabbit Polyclonal anti-CXCR4 antibody (ABIN499698) specifically detects CXCR4 in WB and EIA. The antibody is reactive with Human and Mouse samples.

Quick Overview for CXCR4 antibody (Extracellular Loop) (ABIN499698)

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCR4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CXCR4 Antibody

Binding Specificity: Extracellular Loop

Specificity: CXCR4 antibody was raised against a peptide corresponding to amino acids 182 to 196 in the second extracellular loop (EL) of human CXCR4.

Purification: DEAE

Immunogen: Human CXCR4 (Extracellular Loop) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: 1/500 to 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for CXCR4

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Alternative Name: CD184 / CXCR4

Background: Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.Synonyms: C-X-C chemokine receptor type 4, CXC-R4, CXCR-4, FB22, Fusin, HM89, LCR1, LESTR, Leukocyte-derived seven transmembrane domain receptor, NPYRL, SDF1 receptor, Stromal cell-derived factor 1 receptor

Gene ID: 7852

NCBI Accession: NP_003458

UniProt: P61073

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events