FLASH antibody (C-Term)

Catalog No. ABIN499842

Product Details anti-FLASH Antibody

Target: FLASH (CASP8AP2) (CASP8 Associated Protein 2 (CASP8AP2))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLASH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: FLASH antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy-terminus of human FLASH which differ from those of mouse by one amino acid.

Purification: Affinity chromatography purified via peptide column

Immunogen: Human FLASH (C-Terminus) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: 0.25 to 0.5 μg/mL. An approximate 220 kDa band can be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for FLASH

Target: FLASH (CASP8AP2) (CASP8 Associated Protein 2 (CASP8AP2))

Alternative Name: CASP8AP2 / FLASH (CASP8AP2 Products)

Background: A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2). FLASH is widely expressed.Synonyms: CASP8-associated protein 2, KIAA1315, RIP25

Gene ID: 9994

UniProt: Q9Y563

Pathways: Chromatin Binding, Positive Regulation of Endopeptidase Activity