GPAM antibody (C-Term)

Catalog No. ABIN499910

The Rabbit Polyclonal anti-GPAM antibody is suitable to detect GPAM in samples from Human, Rat and Mouse. It has been validated for WB, IHC (p) and EIA.

Quick Overview for GPAM antibody (C-Term) (ABIN499910)

Target: GPAM (Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPAM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-GPAM Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to GPAT1.

Purification: Affinity chromatography purified via peptide column

Immunogen: GPAT1 antibody was raised against a 15 amino acid peptide near the carboxy terminus of the human GPAT1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: 1 - 2 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for GPAM

Target: GPAM (Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM))

Alternative Name: GPAM / GPAT1

Background: Glycerol-3-phosphate acyltransferase 1 (GPAT1), one of four known GPAT isoforms, is located on the mitochondrial outer membrane, allowing reciprocal regulation with carnitine palmitoyltransferase-1. It is thought to be critical for the development of hepatic steatosis, steatosis triggered by GPAT1 overexpression leads to hepatic and possibly peripheral insulin resistance. GPAT1 is transcriptionally upregulated by insulin and sterol regulatory element binding protein (SREBP-1) and downregulated by AMP-activated protein kinase. Mice deficient in GPAT1 exhibit decreased triacylglycerol (TAG) in cardiomyocytes even in high-fat diets, suggesting that GPAT1 contributes significantly to TAG accumulation in heart tissue during lipogenic or high fat diets. At least two isoforms of GPAT1 are known to exist.Synonyms: Glycerol-3-phosphate acyltransferase 1, KIAA1560

Gene ID: 57678

NCBI Accession: NP_065969

UniProt: Q9HCL2

Pathways: Activated T Cell Proliferation