HRK antibody (Center)

Catalog No. ABIN499950

The Rabbit Polyclonal anti-HRK antibody (ABIN499950) specifically detects HRK in WB and EIA. The antibody is reactive with Human and Mouse samples.

Quick Overview for HRK antibody (Center) (ABIN499950)

Target: HRK (Harakiri, BCL2 Interacting Protein (Contains Only BH3 Domain) (HRK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRK antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-HRK Antibody

Binding Specificity: Center

Specificity: This antibody recognizes HRK.

Purification: Peptide Affinity Chromatography

Immunogen: HRK antibody was raised against a 15 amino acid peptide from near the center of Human Hrk.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: 2.5-5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for HRK

Target: HRK (Harakiri, BCL2 Interacting Protein (Contains Only BH3 Domain) (HRK))

Alternative Name: Harakiri / HRK

Background: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family of proteins, was also identified as novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL. Despite its predicted molecular weight, Hrk often migrates at 12-15 kDa.Synonyms: Activator of apoptosis harakiri, BH3-interacting domain-containing protein 3, BID3, Neuronal death protein DP5

Gene ID: 8739

NCBI Accession: NP_003797

UniProt: O00198