IRAK2 antibody (C-Term)

Catalog No. ABIN500038

Product Details anti-IRAK2 Antibody

Target: IRAK2 (Interleukin-1 Receptor-Associated Kinase 2 (IRAK2))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRAK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: IRAK2 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy-terminus of mouse IRAK2a which is common to all four isoforms. mIRAK2 antibody will also recognize human IRAK2. Anti-IRAK2 has no cross response to IRAK. Anti-IRAK2 will also recognize human IRAK2.

Purification: Affinity chromatography purified via peptide column

Immunogen: Human IRAK2 (C-Terminus) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: 0.5 - 1 μg/mL. A 65 kDa band should be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for IRAK2

Target: IRAK2 (Interleukin-1 Receptor-Associated Kinase 2 (IRAK2))

Alternative Name: IRAK2 (IRAK2 Products)

Background: The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kappaB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family has been identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kappaB activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-?B activation (2,3). Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. Unlike human IRAK2, murine IRAK2 exists as four alternately spliced isoforms (IRAK2a-d), with two isoforms (IRAK2c and d) acting in an inhibitory fashion (4). IRAK2 is expressed in a variety of tissues.Synonyms: IRAK-2, Interleukin-1 receptor-associated kinase-like 2

Gene ID: 108960

UniProt: Q8CFA1

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades