IRGC antibody (C-Term)

Catalog No. ABIN500066

The Rabbit Polyclonal anti-IRGC antibody is suitable to detect IRGC in samples from Human, Rat and Mouse. It has been validated for WB, IF and EIA.

Quick Overview for IRGC antibody (C-Term) (ABIN500066)

Target: IRGC (Immunity-Related GTPase Family, Cinema (IRGC))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRGC antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-IRGC Antibody

Binding Specificity: C-Term

Specificity: This antibody detects IRGC at C-term. It will recognize both isoforms.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: IRGC antibody was raised against a 12 amino acid peptide near the carboxy terminus of human IRGC.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for IRGC

Target: IRGC (Immunity-Related GTPase Family, Cinema (IRGC))

Alternative Name: IRGC

Background: Immunity-related GTPases (IRG) (also known as p47 GTPases) are a family of GTPase proteins found in vertebrates, which play critical roles in mediating innate resistance to intracellular pathogens. IRG genes have been found in a number of mammals and lower species including mice, rats, zebrafish and humans. Most of the mouse genes contain interferon-stimulated response elements which mediate transcriptional activation by IFNs. In humans, only two IRG genes have been found: human IRGC encodes a full-length IRG protein that, like the mouse homologue, is constitutively expressed in testis, while human IRGM encodes a considerably truncated protein that is constitutively expressed in cultured cells including some macrophage cell lines. As the two human genes IRGC and IRGM are not subject to IFN control, it has been suggested that the host resistance mechanism supported by IRG proteins in the mouse is lacking in humans. Two isoforms of IRGC are known to exist.Synonyms: Cinema 1, IIGP5, IRGC1, Immunity-related GTPase cinema 1, Interferon-inducible GTPase 5

Gene ID: 56269

NCBI Accession: NP_062558

UniProt: Q6NXR0