KDM3A antibody (N-Term)

Catalog No. ABIN500074

This Rabbit Polyclonal antibody specifically detects KDM3A in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for KDM3A antibody (N-Term) (ABIN500074)

Target: KDM3A (Lysine (K)-Specific Demethylase 3A (KDM3A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-KDM3A Antibody

Binding Specificity: N-Term

Specificity: This antibody detects KDM3A / JHDM2A. It will not cross-react with JMJD1B or JMJD1C.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: JMJD1A antibody was raised against a 16 amino acid peptide from near the amino terminus of human JMJD1A.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for KDM3A

Target: KDM3A (Lysine (K)-Specific Demethylase 3A (KDM3A))

Alternative Name: KDM3A / JHDM2A

Background: The jumonji domain containing 1A protein (JMJD1A) was originally discovered as a testes specific gene, but has been found to be expressed in numerous tissues. JMJD1A is a histone demethylase and specifically demethylates mono- and dimethyl-H3K9. It has also been found to be a novel interaction partner with ER71, a transcription factor expressed in the testes of adult mice and during embryogenesis. JMJD1A is also a downstream gene of STAT3, a protein that has been found to be important in the maintenance of mouse embryonic stem (mES) cells, and decreased JMJD1A expression correlated with the differentiation of cultured mES cells following the removal of the cytokine LIF. These findings suggest that JMJD1A might be a critical signaling molecule underlying the maintenance of pluripotency in embryonic stem cells. At least two isoforms of JMJD1A are known to exist.Synonyms: JMJD1, JMJD1A, JmjC domain-containing histone demethylation protein 2A, Jumonji domain-containing protein 1A, KIAA0742, Lysine-specific demethylase 3A, TSGA

Gene ID: 55818

NCBI Accession: NP_060903

UniProt: Q9Y4C1

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Warburg Effect