Cytochrome C antibody

Catalog No. ABIN5000922

This Mouse Monoclonal antibody specifically detects Cytochrome C in WB, IHC (p) and IF (p). It exhibits reactivity toward Human, Rat and Rabbitand has been mentioned in 1 publication.

Quick Overview for Cytochrome C antibody (ABIN5000922)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivity: Human, Rat, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytochrome C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Clone: 6B10

Product Details anti-Cytochrome C Antibody

Cross-Reactivity: Human, Rabbit, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant human Cytochrome C Protein

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Cytochrome C antibody (ABIN5000922)

Ding, Chen, Wu, Chen, Zhang, Fan, Wang, Ji, Xie, Ning, Shen: "Increased expression of HERPUD1 involves in neuronal apoptosis after intracerebral hemorrhage." in: Brain research bulletin, Vol. 128, pp. 40-47, (2016) (PubMed).

Target Details for Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Alternative Name: Cytochrome C

Background:

Synonyms: Cytochrome c, CYCS

Background: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

Gene ID: 54205

UniProt: P99999

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity