LIN28A antibody (C-Term)

Catalog No. ABIN500176

This Rabbit Polyclonal antibody specifically detects LIN28A in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for LIN28A antibody (C-Term) (ABIN500176)

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIN28A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-LIN28A Antibody

Binding Specificity: C-Term

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Peptide affinity chromatography

Immunogen: Lin28 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human Lin28.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for LIN28A

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Alternative Name: LIN28

Background: Lin28 is a transcription factor that was first identified through its key role in the pathway of developmental timing in C. elegans. The role of Lin28 in development suggested that it might be useful in the creation of stem cells that might be beneficial in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing Lin28 in addition to the transcription factors POU5F1, Sox2, and NANOG in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing Lin28, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.Synonyms: CSDD1, LIN-28, LIN28A, Lin-28 homolog A, ZCCHC1

Gene ID: 79727

UniProt: Q9H9Z2

Pathways: Stem Cell Maintenance