MED4 antibody (C-Term)

Catalog No. ABIN500270

The Rabbit Polyclonal anti-MED4 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect MED4 in samples from Human, Rat and Mouse.

Quick Overview for MED4 antibody (C-Term) (ABIN500270)

Target: MED4 (Mediator Complex Subunit 4 (MED4))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-MED4 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects MED4. It only recognizes the largest isoform.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: MED4 antibody was raised against a 17 amino acid peptide from near the carboxy terminus of human MED4.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for MED4

Target: MED4 (Mediator Complex Subunit 4 (MED4))

Alternative Name: MED4

Background: The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED4. This protein has also been shown to be part of the vitamin D receptor interacting complex. MED4 was recently identified as an HIV dependency factor (HDF), suggesting that MED4 may be an important drug target in HIV treatment. At least three isoforms of MED4 are known to exist.Synonyms: ARC36, Activator-recruited cofactor 36 kDa component, DRIP36, HSPC126, Mediator complex 4, Mediator of RNA polymerase II transcription subunit 4, TRAP/SMCC/PC2 subunit p36 subunit, VDRIP, Vitamin D3 receptor-interacting protein complex 36 kDa component

Gene ID: 29079

NCBI Accession: NP_054885

UniProt: Q9NPJ6

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha