Occludin antibody (C-Term)

Catalog No. ABIN500409

Product Details anti-Occludin Antibody

Target: Occludin (OCLN)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Occludin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detectc OCLN.

Purification: Peptide affinity chromatography

Immunogen: OCLN antibody was raised against a 15 amino acid peptide from near the carboxy terminus of human OCLN.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

References for anti-Occludin antibody (ABIN500409)

Liang, Zhang, Deng, He, Liang, Liang, Chen, Tang, Chen, Yang: "The Potential Effect of Chinese Herbal Formula Hongqijiangzhi Fang in Improving NAFLD: Focusing on NLRP3 Inflammasome and Gut Microbiota." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2018, pp. 5378961, (2018) (PubMed).

Zhang, Tang, Deng, Chen, Liang, Xie, He, Chen, Yang: "Effects of shenling baizhu powder herbal formula on intestinal microbiota in high-fat diet-induced NAFLD rats." in: Biomedicine & pharmacotherapy, Vol. 102, pp. 1025-1036, (2018) (PubMed).

Target Details for Occludin

Target: Occludin (OCLN)

Alternative Name: Occludin (OCLN Products)

Synonyms: AI503564 antibody, Ocl antibody, ocln antibody, oclnb antibody, tpmt antibody, OCLN antibody, wu:fd23h10 antibody, wu:fi13c01 antibody, zgc:113992 antibody, zgc:56359 antibody, BLCPMG antibody, occludin antibody, occludin S homeolog antibody, occludin a antibody, Ocln antibody, OCLN antibody, ocln.S antibody, ocln antibody, oclna antibody

Background: Tight junctions act as a semi-permeable barrier to the transport of ions, solutes, and water and are considered to function as a fence that divides apical and basolateral domains of plasma membranes. Tight junctions coordinate a variety of signaling and trafficking molecules regulating cell differentiation, proliferation, and polarity and contain a number of junctional proteins including Occludin, Claudins, junctional adhesion molecules (JAMs), as well as multiple scaffold proteins. Occludin, the first identified component of tight junction strands, is thought function as a signal transmitter in multiple signaling pathways and can associate with multiple kinases and phosphatases such as PI3-kinase and protein phosphatases 1 and 2A. At least two isoforms of OCLN are known to exist.Synonyms: OCLN

Gene ID: 4950

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C