PDCD4 antibody (C-Term)

Catalog No. ABIN500461

This Rabbit Polyclonal antibody specifically detects PDCD4 in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PDCD4 antibody (C-Term) (ABIN500461)

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDCD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PDCD4 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects PDCD4 at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affiinity chromatography

Immunogen: PDCD4 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human PDCD4.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for PDCD4

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Alternative Name: PDCD4

Background: Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. During this process, the expression patterns of numerous genes are radically altered. One such gene is the programmed cell death protein 4 (PDCD4), whose expression was found to be upregulated in all cell lines following the onset of apoptosis. PDCD4 encodes a tumor suppressor protein whose expression is lost in carcinomas of breast, colon, lung and prostate. It can bind to and inhibit the helicase activity of the eukaryotic translation initiation factor 4A (4) and inhibit the transactivation and transformation mediated by the transcription factor AP-1. The kinase Akt regulates PDCD4 by phosphorylation, decreasing the ability of PDCD4 to interfere with the transactivation of AP-1-responsive promoter by c-Jun. There are two known isoforms of PDCD4.Synonyms: H731, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Programmed cell death protein 4

Gene ID: 27250

NCBI Accession: NP_663314

UniProt: Q53EL6