POFUT1 antibody (N-Term)

Catalog No. ABIN500521

This Rabbit Polyclonal antibody specifically detects POFUT1 in WB, IHC (p) and EIA. It exhibits reactivity toward Human and Mouseand has been mentioned in 1 publication.

Quick Overview for POFUT1 antibody (N-Term) (ABIN500521)

Target: POFUT1 (Protein O-Fucosyltransferase 1 (POFUT1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POFUT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-POFUT1 Antibody

Binding Specificity: N-Term

Specificity: This antibody detects POFUT1. It is predicted to not cross-react with POFUT2.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse

Purification: Peptide affinity chromatography

Immunogen: POFUT1 antibody was raised against a 16 amino acid peptide from near the amino terminus of human POFUT1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: .5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

References for anti-POFUT1 antibody (ABIN500521)

Takeuchi, Wong, Schneider, Freeze, Takeuchi, Berardinelli, Ito, Lee, Nelson, Haltiwanger: "Variant in human POFUT1 reduces enzymatic activity and likely causes a recessive microcephaly, global developmental delay with cardiac and vascular features." in: Glycobiology, Vol. 28, Issue 5, pp. 276-283, (2018) (PubMed).

Target Details for POFUT1

Target: POFUT1 (Protein O-Fucosyltransferase 1 (POFUT1))

Alternative Name: POFUT1

Background: POFUT1, an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family, adds O-fucose through an O-glycosidic linkage to conserved serine or threonines in epidermal growth factor-like repeats of several cell surface and secreted proteins. Unlike its homolog POFUT2, POFUT1 can also catalyze the transfer of fucose to thrombospondin type 1 repeats. Many of the substrates of POFUT1 are involved in ligand-induced receptor signaling. One such protein is Notch, mouse ES cells lacking POFUT have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or exhibit Notch signaling. At least two isoforms of POFUT1 are known to exist.Synonyms: FUT12, GDP-fucose protein O-fucosyltransferase 1, KIAA0180, O-FucT-1, Peptide-O-fucosyltransferase 1

Gene ID: 23509

NCBI Accession: NP_056167

UniProt: Q9H488

Pathways: Notch Signaling, SARS-CoV-2 Protein Interactome