RHEB antibody (Center)

Catalog No. ABIN500597

The Rabbit Polyclonal anti-RHEB antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect RHEB in samples from Human, Mouse and Rat.

Quick Overview for RHEB antibody (Center) (ABIN500597)

Target: RHEB (Ras Homolog Enriched in Brain (RHEB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHEB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-RHEB Antibody

Binding Specificity: Center

Specificity: This antibody detects Rheb at center.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: Rheb antibody was raised against a 15 amino acid peptide from the middle region of human Rheb.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 μg/mL. Immunohistochemistry on paraffin sections
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for RHEB

Target: RHEB (Ras Homolog Enriched in Brain (RHEB))

Alternative Name: RHEB

Background: Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure (1). While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway (2). Biochemical and genetic studies demon-strate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway (3-5). TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2 (3).Synonyms: GTP-binding protein Rheb, RHEB2, Ras homolog enriched in brain

Gene ID: 6009

UniProt: Q15382

Pathways: RTK Signaling