RUSC1 antibody (C-Term)

Catalog No. ABIN500648

The Rabbit Polyclonal anti-RUSC1 antibody is suitable to detect RUSC1 in samples from Human, Mouse and Rat. It has been validated for WB and EIA.

Quick Overview for RUSC1 antibody (C-Term) (ABIN500648)

Target: RUSC1 (RUN and SH3 Domain Containing 1 (RUSC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUSC1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-RUSC1 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects NESCA.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: RUSC1 antibody was raised against a 17 amino acid peptide from near the carboxy terminus of human RUSC1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for RUSC1

Target: RUSC1 (RUN and SH3 Domain Containing 1 (RUSC1))

Alternative Name: NESCA

Background: RUSC1, also known as NESCA, shares with the related protein RUSC2 a common domain structure of RUN, leucine zipper and SH3 domain in addition to over 30 % amino acid identity. RUSC1 is an adapter protein that can bind to the TrkA receptor and is necessary in the NGF-induced neurite growth of PC12 cells. RUSC1 has also been shown to interact with IkappaB kinase- (IKK-) gamma, the regulatory subunit of the IKK complex that is required for NF-kappaB activation in many signaling pathways such as TNF-R or the TLR pathways. RUSC1 can also bind to the E3 ubiquitin ligase TRAF6, which then catalyzes RUSC1 polyubiquitination. Since overexpression of RUSC1 strongly inhibits TRAF6-mediated polyubiquitination of IKK-gamma, RUSC1 may be a link in the IKK-gamma-mediated NF-kappaB activation pathway.Synonyms: RUN and SH3 domain-containing protein 1, RUSC1

Gene ID: 23623

NCBI Accession: NP_001098673

UniProt: Q9BVN2