SIGLEC11 antibody (C-Term)

Catalog No. ABIN500714

Product Details anti-SIGLEC11 Antibody

Target: SIGLEC11 (Sialic Acid Binding Ig-Like Lectin 11 (SIGLEC11))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIGLEC11 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects Siglec11. It will not cross-react with Siglec10. Despite its predicted size, Siglec11 migrates at a higher than expected molecular weight in SDS-PAGE.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Peptide affinity chromatography

Immunogen: Siglec11 antibody was raised against an 18 amino acid peptide from near the carboxy terminus of human Siglec11.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for SIGLEC11

Target: SIGLEC11 (Sialic Acid Binding Ig-Like Lectin 11 (SIGLEC11))

Alternative Name: SIGLEC11 (SIGLEC11 Products)

Synonyms: sialic acid binding Ig like lectin 11 antibody, SIGLEC11 antibody

Background: Siglecs are sialic acid-binding lectins of the immunoglobulin superfamily that are mainly expressed in cells of the hematopoietic system. Siglec11 is unlike other siglecs in that it binds specifically to a2-8-linked sialic acids and is not found in peripheral blood leukocytes but instead on macrophages of various tissues. Siglec11 is highly homologous to Siglec10 over their extracellular domain but not over their cytoplasmic domain, suggesting that Siglec11 arose through gene duplication followed by a recombination event involving another ancestral siglec gene. Following treatment of Siglec11-transfected cells with pervanadate, Siglec11 becomes tyrosine-phosphorylated and strongly associates with SHP-1 and SHP-2. At least five isoforms of Siglec11 are known to exist.Synonyms: SIGLEC-11, Sialic acid-binding Ig-like lectin 11, Sialic acid-binding lectin 11, UNQ9222/PRO28718

Gene ID: 114132

NCBI Accession: NP_443116

UniProt: Q96RL6