SKA3 antibody (C-Term)

Catalog No. ABIN500732

The Rabbit Polyclonal anti-SKA3 antibody is suitable to detect SKA3 in samples from Human, Rat and Mouse. It has been validated for WB and EIA.

Quick Overview for SKA3 antibody (C-Term) (ABIN500732)

Target: SKA3 (Spindle and Kinetochore Associated Complex Subunit 3 (SKA3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKA3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-SKA3 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects SKA3.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: SKA3 antibody was raised against a 13 amino acid peptide from near the carboxy terminus of human SKA3.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for SKA3

Target: SKA3 (Spindle and Kinetochore Associated Complex Subunit 3 (SKA3))

Alternative Name: SKA3

Background: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Like with SKA1 or SKA2, depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged a metaphase-like state. These SKA3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, suggesting the SKA complex plays a key role in spindle checkpoint silencing and the maintenance of chromosome cohesion in mitosis.Synonyms: C13orf3, RAMA1, Spindle and kinetochore-associated protein 3

Gene ID: 221150

NCBI Accession: NP_659498

UniProt: Q8IX90