SLPI antibody (Center)

Catalog No. ABIN500774

Product Details anti-SLPI Antibody

Target: SLPI (Secretory Leukocyte Peptidase Inhibitor (SLPI))

Binding Specificity: Center

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLPI antibody is un-conjugated

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Specificity: This antibody detects Antileukoproteinase (ALP).

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse

Purification: Peptide affinity chromatography

Immunogen: SLPI antibody was raised against a 17 amino acid peptide from near the center of human SLPI.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

References for anti-SLPI antibody (ABIN500774)

Sayers, Brooks, Sayers, Chertov: "Increased secretory leukocyte protease inhibitor (SLPI) production by highly metastatic mouse breast cancer cells." in: PLoS ONE, Vol. 9, Issue 8, pp. e104223, (2015) (PubMed).

Target Details for SLPI

Target: SLPI (Secretory Leukocyte Peptidase Inhibitor (SLPI))

Alternative Name: Antileukoproteinase (ALP) (SLPI Products)

Synonyms: ALK1 antibody, ALP antibody, BLPI antibody, HUSI antibody, HUSI-I antibody, MPI antibody, WAP4 antibody, WFDC4 antibody, MATN4 antibody, SLPI antibody, ckpi antibody, secretory leukocyte peptidase inhibitor antibody, antileukoproteinase antibody, SLPI antibody, Slpi antibody, LOC102174044 antibody

Background: Secretory leukocyte protease inhibitor (SLPI) is produced at mucosal surfaces, primarily the upper respiratory tract and is thought to play an important role in the antiprotease defense mechanism of the lung. SLPI forms inhibitory complexes with numerous proteolytic enzymes such as neutrophil elastase, and has been shown to possess anti-inflammatory, anti-viral, and antibacterial activities. Its expression in oral epithelial cells is stimulated by HIV-1 gp120, suggesting that SLPI is a component of the oral mucosal response to HIV-1. In peripheral blood monocytes, SLPI can inhibit NF-kB activation by inhibiting IkB degradation in the cytoplasm and competing for NF-kB binding sites in the nucleus. This attenuation of the inflammatory response may also act to suppress liver metastases and other cancer cell invasions, but promote blood-borne metastasis via an invasion-independent pathway.Synonyms: BLPI, HUSI-1, Mucus proteinase inhibitor, Protease inhibitor WAP4, SLPI, Secretory leukocyte protease inhibitor, Seminal proteinase inhibitor, WAP four-disulfide core domain protein 4, WAP4, WFDC4

Gene ID: 6590

UniProt: P03973