NOS1 antibody (pSer1417) (AbBy Fluor® 680)

Catalog No. ABIN5007740

The AbBy Fluor® 680-conjugated Rabbit Polyclonal anti-NOS1 antibody (ABIN5007740) specifically detects NOS1 in WB, IF (cc) and IF (p). The antibody is reactive with Human, Rat and Xenopus laevis samples.

Quick Overview for NOS1 antibody (pSer1417) (AbBy Fluor® 680) (ABIN5007740)

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reactivity: Human, Rat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOS1 antibody is conjugated to AbBy Fluor® 680

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-NOS1 Antibody (AbBy Fluor® 680)

Binding Specificity: pSer1417

Cross-Reactivity: Human, Rat, Xenopus laevis

Predicted Reactivity: Mouse,Zebrafish

Purification: Purified by Protein A.

Immunogen: KLH conjugated Synthesised phosphopeptide derived from human nNOS around the phosphorylation site of Ser1417

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Alternative Name: nNOS

Background:

Synonyms: MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, CASP9

Background: Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Gene ID: 842

UniProt: P55211

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction