SOCS1 antibody (C-Term)

Catalog No. ABIN500788

This Rabbit Polyclonal antibody specifically detects SOCS1 in WB and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SOCS1 antibody (C-Term) (ABIN500788)

Target: SOCS1 (Suppressor of Cytokine Signaling 1 (SOCS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOCS1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-SOCS1 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects SOCS1 / JAB at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide afdfinity chromatography

Immunogen: SOCS1 antibody was raised against a 15 amino acid peptide from near the carboxy terminus of human SOCS1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for SOCS1

Target: SOCS1 (Suppressor of Cytokine Signaling 1 (SOCS1))

Alternative Name: SOCS1

Background: The Suppressor of cytokine signaling (SOCS) and cytokine-inducible SH2 proteins are a family of intracellular proteins which regulate the immune cell responses to cytokines (1). SOCS1 acts to suppress dendritic cell (DC) as well as T cell hyperactivation following cytokine signaling by inhibiting JAK tyrosine kinase, a kinase necessary for type I and II cytokine receptors to initiate signaling, by directly binding to the catalytic domain of the kinase (2). SOCS1 also possesses E3 ubiquitin protein ligase activity that results in the polyubiquitination of its target proteins and subsequent degradation by the proteosome (3). It is through this method that SOCS1 negatively regulates signaling by Toll-like receptors TLR2 and TLR4 by mediating the degradation of the TLR signaling adaptor protein TIRAP (4).Synonyms: JAB, JAK-binding protein, SOCS-1, SSI-1, SSI1, STAT-induced STAT inhibitor 1, Suppressor of cytokine signaling 1, TIP-3, TIP3, Tec-interacting protein 3

Gene ID: 8651

Pathways: JAK-STAT Signaling, Interferon-gamma Pathway, TLR Signaling, Response to Growth Hormone Stimulus