ADAM17 antibody (C-Term)

Catalog No. ABIN500861

The Rabbit Polyclonal anti-ADAM17 antibody has been validated for WB, IF and EIA. It is suitable to detect ADAM17 in samples from Human, Rat and Mouse. There is 1 publication available.

Quick Overview for ADAM17 antibody (C-Term) (ABIN500861)

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAM17 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-ADAM17 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects CD156b / ADAM17 at C-term. 80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated CD156b.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat.

Purification: Immunoaffinity Chromatography

Immunogen: A peptide corresponding to amino acids near the carboxy terminus of Human CD156b / ADAM17

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1: 500 to 1: 2000. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-ADAM17 antibody (ABIN500861)

Wang, Wang, Yin, Zhang, Yu, Kong, Yuan, Fang, Liu, Liu, Shi: "Reduced neurotrophic factor level is the early event before the functional neuronal deficiency in high-fat diet induced obese mice." in: Metabolic brain disease, Vol. 32, Issue 1, pp. 247-257, (2018) (PubMed).

Target Details for ADAM17

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Alternative Name: CD156b / ADAM17

Background: Tumor-necrosis factor-a is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-alpha is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-alpha. TNF-alpha converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFa, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP) (4,5). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.Synonyms: CSVP, Disintegrin and metalloproteinase domain-containing protein 17, Snake venom-like protease, TACE, TNF-alpha convertase, TNF-alpha-converting enzyme

Gene ID: 6868

NCBI Accession: NP_003174

Pathways: Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus