DCAF12 antibody (C-Term)

Catalog No. ABIN500873

The Chicken Polyclonal anti-DCAF12 antibody is suitable to detect DCAF12 in samples from Human, Mouse and Rat. It has been validated for WB and EIA.

Quick Overview for DCAF12 antibody (C-Term) (ABIN500873)

Target: DCAF12 (DDB1 and CUL4 Associated Factor 12 (DCAF12))

Reactivity: Human, Mouse, Rat

Host: Chicken

Clonality: Polyclonal

Conjugate: This DCAF12 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-DCAF12 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects DCAF12.

Purification: Peptide affinity chromatography

Immunogen: TCC52 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human TCC52.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DCAF12

Target: DCAF12 (DDB1 and CUL4 Associated Factor 12 (DCAF12))

Alternative Name: DCAF12

Background: Cancer-testis (CT) antigens are a type of antigen whose expression is highly restricted, primarily in normal testis, but aberrantly in different tumor types. Their immunogenicity in cancer patients makes them prime targets for immunotherapy of human tumors. One such CT antigen is TCC52, a novel centrosomal protein. TCC52 is a WD repeat-containing protein that interacts with the COP9 signalosome, a macromolecular complex that interacts with cullin-RING E3 ligases and regulates their activity by hydrolyzing cullin-Nedd8 conjugates. The normal biological function of this protein is not known at this time.Synonyms: Centrosome-related protein TCC52, DDB1- and CUL4-associated factor 12, KIAA1892, TCC52, Testis cancer centrosome-related protein, WD repeat-containing protein 40A, WDR40A

Gene ID: 25853

NCBI Accession: NP_056212