RCHY1 antibody (AA 31-130) (AbBy Fluor® 680)

Catalog No. ABIN5008857

This Rabbit Polyclonal antibody specifically detects RCHY1 in WB, IF (cc) and IF (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for RCHY1 antibody (AA 31-130) (AbBy Fluor® 680) (ABIN5008857)

Target: RCHY1 (Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RCHY1 antibody is conjugated to AbBy Fluor® 680

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-RCHY1 Antibody (AbBy Fluor® 680)

Binding Specificity: AA 31-130

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PIRH2

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RCHY1

Target: RCHY1 (Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1))

Alternative Name: PIRH2

Background:

Synonyms: ZCHY, ARNIP, CHIMP, PIRH2, RNF199, ZNF363, PRO1996, RING finger and CHY zinc finger domain-containing protein 1, Androgen receptor N-terminal-interacting protein, CH-rich-interacting match with PLAG1, E3 ubiquitin-protein ligase Pirh2, RING finger protein 199, Zinc finger protein 363, p53-induced RING-H2 protein, hPirh2, RCHY1

Background: Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, P73, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Monoubiquitinates the translesion DNA polymerase POLH. Contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity.

Gene ID: 25898

UniProt: Q96PM5