CD248 antibody (N-Term)

Catalog No. ABIN500887

This Rabbit Polyclonal antibody specifically detects CD248 in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CD248 antibody (N-Term) (ABIN500887)

Target: CD248 (CD248 Molecule, Endosialin (CD248))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD248 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-CD248 Antibody

Binding Specificity: N-Term

Specificity: This antibody detects CD248 / TEM1 at N-term. It recognizes only the larger isoform.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat

Purification: Peptide Affinity Chromatography

Immunogen: A 14 amino acid peptide near the amino terminus of the Human TEM1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS, 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD248

Target: CD248 (CD248 Molecule, Endosialin (CD248))

Alternative Name: CD248 / TEM1

Background: Tumor endothelial marker (TEM) 1 was originally identified as a human embryonic fibroblast-specific antigen and was later determined to be endosialin, a single-pass transmembrane glycoprotein that has multiple extracellular domains, including three EGF-like domains, a sushi-like domain, and a C lectin-like domain. TEM proteins are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. While TEM1 is not required for angiogenesis during fetal development, postnatal growth or wound healing, it plays a role in tumor growth, invasion, and metastasis. Fibronectin and collagen types I and IV act as specific ligands of TEM1, leading to suggestions that these molecules may cause changes in the extracellular matrix, cell adhesion and migration during tumor invasion. At least two isoforms of TEM1 are known to exist.Synonyms: CD164L1, Endosialin, Tumor endothelial marker 1

Gene ID: 57124

NCBI Accession: NP_065137