TLR4 antibody (N-Term)

Catalog No. ABIN500952

This anti-TLR4 antibody is a Rabbit Polyclonal antibody detecting TLR4 in WB, IF and EIA. Suitable for Human.

Quick Overview for TLR4 antibody (N-Term) (ABIN500952)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLR4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-TLR4 Antibody

Binding Specificity: N-Term

Specificity: This antibody detects CD284 / TLR4 at N-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ion exchange chromatography

Immunogen: TLR4 antibody was raised against a peptide corresponding to 15 amino acids near the amino-terminus of human TLR4.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 2 to 5 μg/mL. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: CD284 / TLR4

Background: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1-3). These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappaB (4-6). Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis (7).Synonyms: Toll-like receptor 4

Gene ID: 7099

NCBI Accession: NP_612564

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins