TRIM5 antibody (Isoform alpha, Middle Region)

Catalog No. ABIN501052

This Rabbit Polyclonal antibody specifically detects TRIM5 in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for TRIM5 antibody (Isoform alpha, Middle Region) (ABIN501052)

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-TRIM5 Antibody

Binding Specificity: Isoform alpha, Middle Region

Specificity: This antibody detects TRIM5 / RNF88 alpha at Center.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Peptide affinity chromatography

Immunogen: TRIM5 alpha antibody was raised against a synthetic peptide corresponding to amino acids near the mid-region of human TRIM5 alpha.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 to 2 μg/mL. A band at approximately 55 kDa can be detected. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for TRIM5

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Alternative Name: TRIM5 / RNF88

Background: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region . TRIM5 has six alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5alpha variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5alpha variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5alpha acts as a natural barrier to cross-species retrovirus transmission.Synonyms: RING finger protein 88, Tripartite motif-containing protein 5

Gene ID: 85363

NCBI Accession: NP_149023

Pathways: Activation of Innate immune Response