TSC1 antibody (both Isoforms, Middle Region)

Catalog No. ABIN501071

The Rabbit Polyclonal anti-TSC1 antibody has been validated for WB, IF and EIA. It is suitable to detect TSC1 in samples from Human, Mouse and Rat.

Quick Overview for TSC1 antibody (both Isoforms, Middle Region) (ABIN501071)

Target: TSC1 (Tuberous Sclerosis 1 (TSC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TSC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-TSC1 Antibody

Binding Specificity: both Isoforms, Middle Region

Specificity: This antibody detects TSC1 / Hamartin at Center.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: TSC1 antibody was raised against a 15 amino acid peptide from the middle region of human TSC1. TSC1 antibody will recognize both isoforms of TSC1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 μg/mL. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for TSC1

Target: TSC1 (Tuberous Sclerosis 1 (TSC1))

Alternative Name: TSC1 / Hamartin

Background: Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1,2). TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high (3). Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro (4), suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth (4,5). A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.Synonyms: KIAA0243, TSC, Tuberous sclerosis 1 protein

Gene ID: 7248

Pathways: RTK Signaling, AMPK Signaling, Regulation of Cell Size, Tube Formation