UCMA antibody (C-Term)

Catalog No. ABIN501098

The Rabbit Polyclonal anti-UCMA antibody is suitable to detect UCMA in samples from Human and Mouse. It has been validated for WB and EIA.

Quick Overview for UCMA antibody (C-Term) (ABIN501098)

Target: UCMA (Upper Zone of Growth Plate and Cartilage Matrix Associated (UCMA))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCMA antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-UCMA Antibody

Binding Specificity: C-Term

Specificity: This antibody detects UCMA.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse

Purification: Affinity chromatography

Immunogen: UCMA antibody was raised against a 16 amino acid peptide near the carboxy terminus of human UCMA.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 2.5 - 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for UCMA

Target: UCMA (Upper Zone of Growth Plate and Cartilage Matrix Associated (UCMA))

Alternative Name: UCMA

Background: UCMA is a secreted cartilage-specific protein that was discovered in a screen for differentially expressed genes in retinoic acid-treated mouse chondrocytes. It was also identified in a human chondrocyte EST screen for candidate genes of skeletal dysplasias. UCMA expression is thought to parallel that of collagen II with its expression decreasing with maturation chrondrocytes mature. UCMA is processed by a furin-like protease into two fragments, an amino-terminal fragment and a carboxy-terminal fragment (UCMA-C). Application of recombinant UCMA-C to primary osteoblasts, mesenchymal stem cells, and MC3T3-E1 pre-osteoblasts interferes with their osteogenic differentiation, but does not affect expression of chondrocyte-specific genes or chondrocyte proliferation, suggesting that UCMA may be involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells. At least two isoforms of UCMA are known to exist.Synonyms: C10orf49, Unique cartilage matrix-associated protein

Gene ID: 221044

NCBI Accession: NP_660357