HMGCL antibody (AA 1-325)

Catalog No. ABIN5013040

The Rabbit Polyclonal anti-HMGCL antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect HMGCL in samples from Mouse.

Quick Overview for HMGCL antibody (AA 1-325) (ABIN5013040)

Target: HMGCL (3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGCL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-HMGCL Antibody

Binding Specificity: AA 1-325

Purpose: Polyclonal Antibody to 3-Hydroxymethyl-3-Methylglutaryl Coenzyme A Lyase (HMGCL)

Specificity: The antibody is a rabbit polyclonal antibody raised against HMGCL. It has been selected for its ability to recognize HMGCL in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPD685Mu01Recombinant 3Hydroxymethyl3Methylglutaryl Coenzyme A Lyase (HMGCL)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for HMGCL

Target: HMGCL (3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL))

Alternative Name: HMGCL

Background: HL, Hydroxymethylglutaricaciduria, Hydroxymethylglutaryl-CoA lyase, mitochondrial