CDC25A antibody (AA 190-484)

Catalog No. ABIN5027104

The Mouse Monoclonal anti-CDC25A antibody has been validated for WB and IHC. It is suitable to detect CDC25A in samples from Human.

Quick Overview for CDC25A antibody (AA 190-484) (ABIN5027104)

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDC25A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: ABM4A46

Product Details anti-CDC25A Antibody

Binding Specificity: AA 190-484

Purification: Protein G Chromatography

Immunogen: A partial length recombinant CDC25A protein (amino acids 190-484) was used as the immunogen for this antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: WB: 2-4 μg/mL, IHC: 5 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA, PH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Target Details for CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Alternative Name: CDC25A

Background: CDC25A is a dual specificity (tyr/thr) phosphatase and a putative upregulated c-Myc target gene. It is a regulator of the cell cycle required for progression from G1 to S phase. CDC25A is a member of the CDC25 family of phosphatases. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. Overexpression of CDC25A bypassed the mechanism of cell cycle arrest, leading to enhanced DNA damage and decreased cell survival. Specific degradation of CDC25A is identified as part of the DNA damage checkpoint mechanism and suggested how CDC25A overexpression in human cancers might contribute to tumorigenesis.

Molecular Weight: 59 kDa

Gene ID: 993

UniProt: P30304

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase