DACT1 antibody (AA 645-820)

Catalog No. ABIN5027132

The Rabbit Polyclonal anti-DACT1 antibody (ABIN5027132) specifically detects DACT1 in WB. The antibody is reactive with Human samples.

Quick Overview for DACT1 antibody (AA 645-820) (ABIN5027132)

Target: DACT1 (Dapper, Antagonist of beta-Catenin, Homolog 1 (DACT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DACT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DACT1 Antibody

Binding Specificity: AA 645-820

Purification: Protein A Chromatography

Immunogen: A partial length recombinant Dapper homolog 1 protein (amino acids 645-820) was used as the immunogen for this antibody.

Isotype: IgG

Application Details

Application Notes: WB: 2-4 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA, PH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Target Details for DACT1

Target: DACT1 (Dapper, Antagonist of beta-Catenin, Homolog 1 (DACT1))

Alternative Name: Dapper homolog 1

Background: Dapper homolog 1 (DACT1) is a cytoplasmic protein and a member of DACT family. It is an important regulator in the planar cell polarity (PCP) pathway. DACT1 interacts and posttranslationally regulates central PCP components Dvl2 and Vangl2 and modulates PCP downstream of the Rac1/JNK cascade. The gene for DACT1 is located at chromosome 14q22.3, which encodes a 836 amino acid protein with a putative leucine zipper (LZ) domain in the amino-terminal end and a consensus PDZ binding (PDZ-B) motif in the carboxy-terminal end that allows the DACT1 protein to interact with the Dishevelled (Dvl) PDZ domain. DACT1 is an important positive regulator in colon cancer through regulating the stability and sublocation of Beta-catenin. The overexpression of DACT1 leads to the increased accumulation of nonphosphorylated Beta-catenin in the cytoplasm and particularly in the nuclei.It interacts with GSK-3beta and beta-catenin and stabilizes it via DACT1-induced effects on GSK-3beta which directly interacts with beta-catenin proteins. This interaction identifies DACT1 as an important positive regulator in colon cancer and suggests a potential strategy for the therapeutic control of the beta-catenin-dependent pathway.

Molecular Weight: 92 kDa

Gene ID: 51339

UniProt: Q9NYF0

Pathways: Positive Regulation of fat Cell Differentiation