HMGB1 antibody (AA 1-200)

Catalog No. ABIN5027246

This anti-HMGB1 antibody is a Mouse Monoclonal antibody detecting HMGB1 in WB, IHC and FACS. Suitable for Human.

Quick Overview for HMGB1 antibody (AA 1-200) (ABIN5027246)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: ABM24D3

Product Details anti-HMGB1 Antibody

Binding Specificity: AA 1-200

Purification: Protein G Chromatography

Immunogen: A partial length recombinant HMGB1 protein (amino acids 1- 200) was used as the immunogen for this antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: WB: 2-4 μg/mL,FACS: 0.5 μg/10^6 cells, IHC: 1 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA, PH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMGB1

Background: HMGB1 (High-mobility group protein B1) is a 25 kDa non-histone chromatin protein, secreted by immune cells through leaderless secretory pathway. HMGB1 supports transcription and also interacts with nucleosomes to loosen packed DNA and remodel the chromatin. The presence of HMGB1 in the nucleus depends on posttranslational modifications. When the protein is not acetylated, it stays in the nucleus, but hyperacetylation on lysine residues causes it to translocate into the cytosol. HMGB1 has the ability to bend DNA in order to facilitate binding of other proteins to the DNA. HMGB1 has one important role in V(D)J recombination where act as a cofactor of the RAG complex. HMGB1 stimulates a plethora of cellular responses like inflammation, attracting inflammatory cells, recruiting stem cells and promoting their proliferation. HMGB1 is highly expressed in human kidney, tonsils, adipose tissue, adrenal cortex and amniotic fluid.

Molecular Weight: 30 kDa

Gene ID: 3146

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome