Dnmt2 antibody

Catalog No. ABIN5027475

This Mouse Monoclonal antibody specifically detects Dnmt2 in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for Dnmt2 antibody (ABIN5027475)

Target: Dnmt2 (TRDMT1) (tRNA Aspartic Acid Methyltransferase 1 (TRDMT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Dnmt2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: ABM1H70

Product Details anti-Dnmt2 Antibody

Cross-Reactivity: Mouse (Murine)

Purification: Protein G Chromatography

Immunogen: A partial length recombinant protein from DNMT2 was used as the immunogen for this antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: WB: 4-6 μg/mL, FACS: 0.5-1 μg/10^6

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA, PH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Target Details for Dnmt2

Target: Dnmt2 (TRDMT1) (tRNA Aspartic Acid Methyltransferase 1 (TRDMT1))

Alternative Name: DNMT2

Background: DNMT2, also known as tRNA-aspartic acid methyltransferase 1 (TRDMT1), is highly conserved cytosine-C5 methyltransferase that introduces the C38 methylation of tRNAAsp in many species, including lower eukaryotes, plants, insects, and humans. DNMT2 contains all 10 sequence motifs that are conserved among m5C MTases, including the consensus S-adenosyl-L-methionine-binding motifs and the active site ProCys dipeptide. It is a relatively small protein of 391 amino acids and lacks the large N-terminal domains present in the DNMT1 and DNMT3 families. DNMT2 is ubiquitously expressed with multiple mRNA species in most human and mouse adult tissues. It is variably expressed in human cancer cell lines.

Molecular Weight: 45 kDa

Gene ID: 1787

UniProt: Q6ICS7