TRIM29 antibody (AA 2-291)

Catalog No. ABIN5027599

The Mouse Monoclonal anti-TRIM29 antibody has been validated for WB and IHC. It is suitable to detect TRIM29 in samples from Human.

Quick Overview for TRIM29 antibody (AA 2-291) (ABIN5027599)

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRIM29 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: ABM43D2

Product Details anti-TRIM29 Antibody

Binding Specificity: AA 2-291

Purification: Protein G Chromatography

Immunogen: A partial length recombinant Trim29 protein (amino acids 2-291) was used as the immunogen for this antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: WB: 2-4 μg/mL, IHC: 1 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA, PH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Target Details for TRIM29

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Alternative Name: Trim29

Background: TRIM29 (Tripartite motif-containing 29) is a member of the tripartite motif (TRIM) family of transcription factors, characterized by the conserved RING finger, B-box, and coiled-coil domains. The TRIM family has been implicated in a variety of physiologic processes including development, apoptosis and epithelial-mesenchymal transition (EMT). Depending on a tumor types, TRIM29 also may function as an oncogene or a tumor suppressor. As an oncogene, it facilitates tumor cell proliferation and invasion through stabilization of Beta-catenin. The mechanisms may also involve deactivation of p53 activity and promoting cell survival by inhibiting proapoptotic genes regulated by p53. As a histone binding protein TRIM29 is responsible for DDR (DNA damage response). It functions as a scaffold protein to assemble DNA repair proteins into chromatin followed by efficient activation of DDR. TRIM29 binds to modified histone H3 and H4 tails in the context of nucleosomes. Furthermore, chromatin binding of TRIM29 is required for the phosphorylation of H2AX nucleosomes and cell viability in response to ionizing radiation.

Molecular Weight: 65 kDa

Gene ID: 23650

UniProt: Q14134

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus