EGLN1 antibody (AA 1-50)

Catalog No. ABIN504713

The Mouse Monoclonal anti-EGLN1 antibody has been validated for WB and IHC. It is suitable to detect EGLN1 in samples from Human.

Quick Overview for EGLN1 antibody (AA 1-50) (ABIN504713)

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EGLN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-EGLN1 Antibody

Binding Specificity: AA 1-50

Cross-Reactivity: Cow (Bovine), Human, Rat (Rattus)

Purification: Aff - Purified

Immunogen: A peptide within AA 1-50 of the human protein. [Swiss-Prot# Q9GZT9].

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: -20 °C

Target Details for EGLN1

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Alternative Name: HIF Prolyl Hydroxylase 2

Background: HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.

UniProt: Q9GZT9

Pathways: cAMP Metabolic Process, Warburg Effect