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Rubicon antibody (N-Term) (Atto 390)

This anti-Rubicon antibody is a Rabbit Polyclonal antibody detecting Rubicon in WB, IHC, IF and ICC. Suitable for Human.
Catalog No. ABIN5066354

Quick Overview for Rubicon antibody (N-Term) (Atto 390) (ABIN5066354)

Target

See all Rubicon (KIAA0226) Antibodies
Rubicon (KIAA0226) (KIAA0226)

Reactivity

  • 29
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
Human

Host

  • 27
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Rabbit

Clonality

  • 27
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Polyclonal

Conjugate

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This Rubicon antibody is conjugated to Atto 390

Application

  • 24
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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
  • Binding Specificity

    • 12
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    N-Term

    Specificity

    Detects ~95 kDa.

    Cross-Reactivity

    Human

    Purification

    Peptide Affinity Purified

    Immunogen

    Synthetic peptide from the N-terminal of Human Rubicon

    Isotype

    IgG
  • Application Notes

    • WB (1:1000)
    • ICC/IF (1:100)
    • IHC (1:50)
    • optimal dilutions for assays should be determined by the user.

    Comment

    A 1:1000 dilution of ABIN5066354 was sufficient for detection of Rubicon in 15 μg of human Hela cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C

    Storage Comment

    Conjugated antibodies should be stored at 4°C
  • Target

    Rubicon (KIAA0226) (KIAA0226)

    Alternative Name

    Rubicon

    Gene ID

    9711

    NCBI Accession

    NP_001139114

    UniProt

    Q92622

    Pathways

    Autophagy
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