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Sulfotyrosine antibody (Atto 390)

WB, ELISA, ICC, IF, IHC Host: Mouse Monoclonal 7C5 Atto 390
Catalog No. ABIN5067488
  • Target See all Sulfotyrosine products
    Sulfotyrosine
    Host
    • 10
    Mouse
    Clonality
    • 10
    Monoclonal
    Conjugate
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Sulfotyrosine antibody is conjugated to Atto 390
    Application
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
    Specificity
    Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.
    Purification
    Protein G Purified
    Immunogen
    Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
    Clone
    7C5
    Isotype
    IgG1
  • Application Notes
    • WB (1:1000)
    • ICC/IF (1:50)
    • ELISA (1:1000)
    • IHC (1:25)
    • optimal dilutions for assays should be determined by the user.
    Comment

    A 1:1000 dilution of ABIN5067488 was sufficient for detection of Sulfotyrosine in 0.5 μg of Sulfotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    Sulfotyrosine
    Abstract
    Sulfotyrosine Products
    Target Type
    Amino Acid
    Background
    Tyrosine sulfation is a common post-translational protein modification catalyzed by tyrosylprotein sulfotransferase (TPST) (1). Sulfotyrosines mediate interactions between hirudin and thrombin, P-selectin glycoprotein ligand-1 and P-selectin, and von Willebrand factor and factor VIII. Tyrosine sulfation of certain receptors is required for several viruses and parasites to enter their hosts.
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