ALOX15 antibody (AA 1-662)

Catalog No. ABIN513270

Cited in 3+ publications. The Mouse Monoclonal anti-ALOX15 antibody (Clone 3D8) (ABIN513270) specifically detects ALOX15 in WB, ELISA and IHC (p). The antibody is reactive with Human samples.

Quick Overview for ALOX15 antibody (AA 1-662) (ABIN513270)

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ALOX15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 3D8

Product Details anti-ALOX15 Antibody

Binding Specificity: AA 1-662

Purpose: Mouse monoclonal antibody raised against a full length recombinant ALOX15.

Sequence: MGLYRIRVST GASLYAGSNN QVQLWLVGQH GEAALGKRLW PARGKETELK VEVPEYLGPL LFVKLRKRHL LKDDAWFCNW ISVQGPGAGD EVRFPCYRWV EGNGVLSLPE GTGRTVGEDP QGLFQKHREE ELEERRKLYR WGNWKDGLIL NMAGAKLYDL PVDERFLEDK RVDFEVSLAK GLADLAIKDS LNVLTCWKDL DDFNRIFWCG QSKLAERVRD SWKEDALFGY QFLNGANPVV LRRSAHLPAR LVFPPGMEEL QAQLEKELEG GTLFEADFSL LDGIKANVIL CSQQHLAAPL VMLKLQPDGK LLPMVIQLQL PRTGSPPPPL FLPTDPPMAW LLAKCWVRSS DFQLHELQSH LLRGHLMAEV IVVATMRCLP SIHPIFKLII PHLRYTLEIN VRARTGLVSD MGIFDQIMST GGGGHVQLLK QAGAFLTYSS FCPPDDLADR GLLGVKSSFY PQDALRLWEI IYRYVEGIVS LHYKTDVAVK DDPELQTWCR EITEIGLQGA QDRGFPVSLQ ARDQVCHFVT MCIFTCTGQH ASVHLGQLDW YSWVPNAPCT MRLPPPTTKD ATLETVMATL PNFHQASLQM SITWQLGRRQ PVMVAVGQHE EEYFSGPEPK AVLKKFREEL AALDKEIEIR NAKLDMPYEY LRPSVVENSV AI

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: ALOX15 (AAH29032, 1 a.a. ~ 662 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-ALOX15 antibody (ABIN513270)

Gregus, Dumlao, Wei, Norris, Catella, Meyerstein, Buczynski, Steinauer, Fitzsimmons, Yaksh, Dennis: "Systematic analysis of rat 12/15-lipoxygenase enzymes reveals critical role for spinal eLOX3 hepoxilin synthase activity in inflammatory hyperalgesia." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 27, Issue 5, pp. 1939-49, (2013) (PubMed).

Dobrian, Lieb, Ma, Lindsay, Cole, Ma, Chakrabarti, Kuhn, Wohlgemuth, Fontana, Nadler: "Differential expression and localization of 12/15 lipoxygenases in adipose tissue in human obese subjects." in: Biochemical and biophysical research communications, Vol. 403, Issue 3-4, pp. 485-90, (2010) (PubMed).

Calandria, Marcheselli, Mukherjee, Uddin, Winkler, Petasis, Bazan: "Selective survival rescue in 15-lipoxygenase-1-deficient retinal pigment epithelial cells by the novel docosahexaenoic acid-derived mediator, neuroprotectin D1." in: The Journal of biological chemistry, Vol. 284, Issue 26, pp. 17877-82, (2009) (PubMed).

Target Details for ALOX15

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Alternative Name: ALOX15

Background: Full Gene Name: arachidonate 15-lipoxygenase
Synonyms: 15-LOX-1,15-LOX2

Gene ID: 246

Pathways: Regulation of Actin Filament Polymerization