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HSP27 antibody (AA 96-205)

The Mouse Monoclonal anti-HSP27 antibody has been validated for WB, ELISA, IF, IP and PLA. It is suitable to detect HSP27 in samples from Human.
Catalog No. ABIN516740

Quick Overview for HSP27 antibody (AA 96-205) (ABIN516740)

Target

See all HSP27 (HSPB1) Antibodies
HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity

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  • 25
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Human

Host

  • 237
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Mouse

Clonality

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  • 125
Monoclonal

Conjugate

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This HSP27 antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Proximity Ligation Assay (PLA)

Clone

3G3
  • Binding Specificity

    • 41
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    • 16
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    AA 96-205

    Purpose

    Mouse monoclonal antibody raised against a partial recombinant HSPB1.

    Sequence

    RVSLDVNHFA PDELTVKTKD GVVEITGKHE ERQDEHGYIS RCFTRKYTLP PGVDPTQVSS SLSPEGTLTV EAPMPKLATQ SNEITIPVTF ESRAQLGGPE AAKSDETAAK

    Cross-Reactivity

    Human

    Characteristics

    Antibody Reactive Against Recombinant Protein.

    Immunogen

    HSPB1 (NP_001531, 96 a.a. ~ 205 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

    Isotype

    IgG2a
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Buffer

    In 1x PBS, pH 7.4

    Handling Advice

    Aliquot to avoid repeated freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Target

    HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

    Alternative Name

    HSPB1

    Background

    Full Gene Name: heat shock 27 kDa protein 1
    Synonyms: CMT2F,DKFZp586P1322,HMN2B,HS.76067,HSP27,HSP28,Hsp25,SRP27

    Gene ID

    3315

    NCBI Accession

    NM_001540

    Pathways

    MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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